Characterization of HS fragments released by heparanase from cell surface and extracellular matrix
Production of recombinant heparanase in milligram amounts
Identification of the active site for heparin/HS for heparanase; isolation and synthesis of the corresponding peptide
Set-up of a mass spectrometric method for studying heparin/HS oligosaccharide fragments generated by heparanase
Preparation of novel building blocks for synthesis of HS oligosaccharides
Conformation of arrays of sulfategroups on different polysaccharide backbones
Synthesis of novel sulfated poly- oligosaccharides
Synthesis of C-C glycomimetics of sulfated oligosaccharides
In vitro screening of novel products and in vivo testing of those more active as heparanase inhibitors
Establishement of heparanase-overexpressing cell lines as tools for studying the involvement of heparanase in angiogenesis and metastasis
Pharmacokinetic studies on selected, fluorescein-labeled heparanase inhibitors
Selection of lead compounds for in-depth in vivo studies